Proteinase K, MB and NGS Grade
- High activity and exceptional purity
- Active at high temperatures and denaturing conditions
- From Parengyodontium album (Tritirachium album)
- Free of exonucleases, endonucleases, and ribonucleases
- Lyophilized or as a solution (MB grade only)
Proteinase K, MB and NGS Grade
Why proteinase K ?
- It is a broad spectrum endopeptidase with a very high specific activity
- It is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS
- It does not compromise the integrity os isolated DNA or RNA during nucleic acid preparations
- It is free of exonucleases, endonucleases, and ribonucleases
- Proteinase K NGS Grade: an extra purification step results in significantly increased solubility (2.5 fold), increased specific activity, and decreased DNA content, compared to ou Molecular Biology Grade product.
Features
- Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris
- High activity and exceptional purity
- Active at high temperatures (up to 56°C) and denaturing conditions
- Stable over a wide pH range: 4.0–12.5 (optimum pH 7.5–8.0)
- Decreased amount of host DNA (≤ 10 pg/mg / MBG or ≤ 0.1 pg/mg NGS)
- Units: One unit of Proteinase K hydrolyzes urea-denaturated hemoglobin producing color equivalent of 1 μmol tyrosine per 1 min at 37°C and pH 7.5 (Folin & Ciocalteu’s method), 1 U = 1 mAnsonU
Applications
- Extraction of DNA and RNA from different starting materials
- Purification of target material from contaminating proteins
- Removal of DNases and RNases during nucleic acids isolation
That's how important proteinase K is
- Proteinase K is a widely used enzyme to digest proteins in various applications such as nucleic acid isolation and purification
- The isolated material is used in a variety of experiments and diagnostic applications
- The quality of this basic enzyme guarantees the success of the whole experiment
Proteinase K MB Grade – Lyophilized powder
Solubility in water: ≥ 20 mg/ml
Activity: ≥ 30 U/mg lyophilizate
≥ 40 U/mg protein
Protein content: ≥ 70% Protein content is determined by measuring absorbance at 280 nm.
DNA content: ≤ 10 pg/mg by qPCR
Proteinase K MB Grade – Solution
Proteinase K concentration: 20 mg/ml
Activity: ≥ 800 U/ml
DNA content: ≤ 0.25 pg/U by qPCR
Proteinase K – Lyophilized powder, NGS Grade
DNA content: ≤ 0.1 pg/mg by qPCR
Solubility in water: ≤ 0.1 pg/mg by qPCR
Activity: ≤ 0.1 pg/mg by qPCR
≥ 45 U/mg protein
Protein content: ≥ 70% Protein content is determined by measuring absorbance at 280 nm.
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LabConsulting GmbH and LC LabService GmbH are Austrian companies which cooperate with over 600 manufacturers and offers a selection of more than 180,000 products and various laboratory product and service solutions for our Labcustomers.
Die LabConsulting GmbH und die LC LabService GmbH sind österreichische Unternehmen, die mit über 600 global verteilten Herstellern zusammenarbeiten und mit einer Auswahl von mehr als 180.000 Produkten und verschiedenen Laborprodukt- und Servicelösungen unseren Parnern im Labor anbieten.
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