NucleoSpin Gel and PCR Clean-up Kit from Macherey-Nagel

High-Efficiency PCR Clean-up and Gel Extraction for DNA/RNA

The NucleoSpin Gel & PCR Clean-up Kit offers a fast and efficient solution for purifying PCR products and extracting DNA/RNA from agarose and polyacrylamide gels. This two-in-one kit simplifies the workflow by using one optimized buffer for both applications, ensuring reliable and high-quality results every time.

Key Features of NucleoSpin Gel & PCR Clean-up

• Dual Application in One Kit: The kit is designed for both PCR clean-up and gel extraction, using one optimized buffer for both applications.
• High DNA Recovery: Achieve high recoveries for small DNA fragments down to 50 bp, making it ideal for a variety of molecular biology applications.
• Concentrated DNA: The minimized elution volume (as low as 15 μL) ensures that you get highly concentrated DNA, suitable for downstream processes such as cloning and sequencing.
• pH Indicator in Binding Buffer: The buffer contains a pH indicator that allows easy visual confirmation of the correct binding conditions, ensuring optimal performance during purification.
• Wide Compatibility: The kit is compatible with all gel buffer systems, including TAE and TBE, and supports both centrifugation and vacuum processing.
• Removes Contaminants Efficiently: Removes enzymes, nucleotides, salts, primers, and other impurities from PCR reactions. Ensures the removal of contaminants like detergents, dyes, and additives commonly used in molecular biology labs.

Applications

The NucleoSpin Gel & PCR Clean-up Kit is suitable for a variety of applications, including:

• Purification of PCR Products: Removes unwanted components such as primers, nucleotides, and enzymes, providing high-purity DNA.
• Extraction of DNA from Gels: Efficient extraction of DNA from agarose and polyacrylamide gels, even for small fragments.
• Downstream Applications: Ideal for cloning, sequencing, PCR, restriction analysis, and other molecular biology processes requiring clean, high-quality DNA.

How It Works

1. PCR Clean-up: Add the binding buffer to your PCR product. The buffer contains a pH indicator to confirm optimal conditions for binding. Apply the mixture to the silica membrane. Wash the sample using the ethanolic wash buffer to remove contaminants such as nucleotides and salts.

2. Gel Extraction: For DNA extraction from gels, dissolve the gel slice in the high-salt binding buffer. Apply the dissolved gel to the column and centrifuge. Contaminants are removed by a washing step.

3. Elution: Pure DNA is eluted in a low ionic strength buffer (slightly alkaline), with a minimized elution volume of 15 μL for highly concentrated DNA.

Why Choose the NucleoSpin Gel and PCR Clean-up Mini Kit?

• Optimized for Speed and Efficiency: Complete your PCR clean-up or gel extraction in just 10 minutes (processing 6 preps at a time), making it an ideal solution for high-throughput laboratories.
• High Recovery Rates for Small Fragments: The kit is optimized for recovering small DNA fragments, as small as 50 bp, ensuring you get the most out of your samples.
• Simple and Reliable: With a pH indicator for easy confirmation of the correct conditions and flexible processing options (centrifugation or vacuum), this kit simplifies your workflow without compromising on quality.

Specifications

• Processing Time: 10 minutes for 6 preps
• Capacity: 250 preps per kit
• Sample Types: Suitable for agarose and polyacrylamide gels
• Elution Volume: Minimized elution volume of 15 μL for highly concentrated DNA
• Purity: Ensures complete removal of contaminants such as nucleotides, primers, enzymes, detergents, and dyes

Ideal for:

• Molecular Biologists: Ideal for applications in molecular biology, genetics, and biotechnology requiring purified DNA/RNA for downstream analysis.
• Clinical Diagnostics: Suitable for DNA/RNA extraction in clinical labs where high-quality genetic material is needed for diagnostic purposes.
• Research Laboratories: Used for research purposes in areas such as gene cloning, sequencing, and expression analysis.

Conclusion

The NucleoSpin Gel & PCR Clean-up Kit provides a fast, efficient, and reliable method for cleaning up PCR products and extracting DNA from gels. Its unique design, high recovery rates, and simplified process make it an essential tool for any molecular biology lab. Whether you’re preparing DNA for cloning, sequencing, or other downstream applications, this kit delivers high-quality, concentrated DNA with minimal effort.

Buy Nucleospin Gel and PCR Clean-up Macherey Nagel

Enhance your lab’s efficiency with the NucleoSpin Gel & PCR Clean-up Kit, a fast and reliable solution for DNA/RNA purification and extraction. Contact LabConsulting today for more information or to request a quote.