SensiFAST™ cDNA Synthesis Kit

SensiFAST™ cDNA Synthesis Kit – Fast, Accurate, and Reliable cDNA Synthesis for qPCR

The SensiFAST™ cDNA Synthesis Kit is a high-performance solution for fast and accurate synthesis of first-strand cDNA from RNA samples. It is designed for gene expression analysis, tissue biopsy studies, and RNA detection. With a novel TransAmp™ buffer system and an optimized blend of random hexamers and anchored oligo(dT) primers, this kit delivers high-quality cDNA ready for real-time qPCR in as little as 5 minutes.

Ideal for researchers working with low-copy or dilute RNA, this kit guarantees accurate detection and reliable results across a wide range of applications. Whether you are working with complex templates, inhibitors, or small sample volumes, the SensiFAST™ cDNA Synthesis Kit provides the speed, sensitivity, and versatility needed for successful cDNA synthesis.

Key Features of SensiFAST™ cDNA Synthesis Kit

• Efficient Reverse Transcription: The high target affinity, coupled with the advanced TransAmp™ Buffer, improves the yield of full-length cDNA, providing reliable results.
• Unbiased cDNA Synthesis: The optimized mix of random hexamers and anchored oligo(dT) primers ensures complete 5’ to 3’ RNA sequence coverage, crucial for precise gene expression analysis.
• Sensitive and Accurate: Detect low-copy targets with lower Ct values from as little as 1 pg of RNA input, ensuring accurate detection even in dilute samples.
• Robust Performance: Achieve consistent reverse transcription under challenging conditions, including complex RNA templates and inhibitors.
• Fast Workflow: Complete high-yield reverse transcription in as little as 5 minutes, ensuring rapid results in real-time qPCR experiments.

Applications

The SensiFAST™ cDNA Synthesis Kit is ideal for a variety of molecular biology applications, including:

• Gene Expression Analysis: Perfect for qPCR studies requiring accurate gene expression quantification from different RNA templates.
• Tissue Biopsy Analysis: Great for reverse transcription from small, precious RNA samples, such as laser-microdissected tissues or biopsy samples.
• miRNA Profiling & Quantification: Enables accurate reverse transcription for miRNA detection and quantification.
• Pathogen Detection: Sensitive enough to detect viral and bacterial RNA, making it useful for clinical diagnostics and pathogen research.
• RNA Target Detection: Ensures reliable detection of RNA targets, even in low-copy or challenging samples.

Why Choose SensiFAST™ cDNA Synthesis Kit?

• High-Quality Reverse Transcription: Achieve high-fidelity cDNA synthesis for downstream applications like qPCR, sequencing, and cloning.
• Broad Application Compatibility: Supports various RNA sample types, including total RNA from tissues, biopsies, and cell lines, making it versatile for different molecular biology needs.
• Fast and Reliable: The ability to generate qPCR-ready cDNA in as little as 5 minutes allows you to proceed with your experiments quickly without compromising on quality.

For more advanced laboratory services and technical support, visit our Lab Service & Technical Support page to learn more about our solutions.

Technical Specifications

• Reactions: 250 reactions per kit
• Input RNA: As low as 1 pg of total RNA
• Reaction Time: Complete reverse transcription in 5-15 minutes
• Primer Mix: Random hexamers and anchored oligo(dT) primers for full RNA sequence representation
• Buffer System: TransAmp™ buffer system for enhanced performance
• RNA Compatibility: Suitable for a wide range of RNA sample types, including tissue biopsies and clinical samples

How It Works

• Lysis and RNA Isolation: Isolate RNA from your samples, such as tissue biopsies, using methods like the NucleoSpin RNA Isolation Kit.
• Reverse Transcription: Add your isolated RNA to the SensiFAST™ cDNA Synthesis Kit. The TransAmp™ buffer ensures optimal conditions for reverse transcription, and LyseControl helps you monitor the reaction visually.
• cDNA Synthesis: In just 5-15 minutes, your RNA is reverse transcribed into high-quality cDNA, ready for use in downstream applications such as qPCR or miRNA profiling.
• Real-Time PCR: Use your synthesized cDNA with SensiFAST Probe or SYBR Kits for fast and accurate real-time qPCR results.

To learn more about our range of lab solutions, check out our Lab Outsourcing Services, where we offer expert management of laboratory operations.