NucleoBond Xtra Midi Kit from Macherey-Nagel

Fast and Efficient Plasmid DNA Purification with NucleoBond Xtra Midi Kit

Why This Kit is Perfect for Your Lab

The NucleoBond Xtra Midi Kit is ideal for quickly purifying plasmid DNA from E. coli cultures, offering high yields and reliable results. With its ability to process up to 50 preparations, this kit is perfect for small and large-scale lab work. It provides up to 400 µg of purified plasmid DNA, making it suitable for applications such as cloning, transfection, and sequencing.

Key Benefits of This NucleoBond Xtra Midi Kit

• Speed and Efficiency: Complete your plasmid DNA extraction in just 70 minutes per preparation, saving valuable time in the lab.
• High Yields: Extract up to 400 µg of plasmid DNA per prep, suitable for a wide range of molecular biology applications.
• Lysate Clarification: A built-in column filter makes lysate clarification easy, preventing clogging and ensuring a smooth process.
• LyseControl Technology: The blue LyseControl reagent visually ensures optimal lysis and reduces contamination risks, providing a higher yield of plasmid DNA.
• Proven Anion Exchange Technology: The patented silica-based resin binds plasmid DNA with high specificity, ensuring clean, high-quality results.
• Optional Speed Enhancement: Use the NucleoSpin Finalizer to reduce the prep time to just 30 minutes for faster results.

How Does NucleoBond Xtra Midi Work?

1. Cell Lysis: Cells are broken down using an optimized buffer. LyseControl turns the solution blue, indicating proper lysis. This helps you avoid contamination from genomic DNA.

2. Binding of Plasmid DNA: The lysate flows through the column, where plasmid DNA binds to the anion exchange resin.

3. Washing: A series of washing steps removes proteins and other impurities.

4. Elution and Collection: Purified DNA is released from the column and can be dissolved in a low-salt buffer or water. Use the NucleoSpin Finalizer to accelerate the process.

5. DNA Ready for Use: The purified plasmid DNA is ready for use in various applications like cloning, sequencing, and gene expression studies.

Applications for High-Quality Plasmid DNA

1. Cloning and Genetic Engineering

Obtain high-quality plasmid DNA that’s perfect for cloning and other genetic engineering projects.

2. Transfection for Gene Expression

Use the clean plasmid DNA in transfection protocols for gene expression and other studies.

3. Sequencing and Analysis

The high-purity DNA makes this kit ideal for next-generation sequencing and other analytical techniques.

4. In Vitro Transcription

Use the purified DNA for in vitro transcription and similar molecular biology applications.

Features That Make This Kit Stand Out

• Simple, Quick Process: Purify plasmid DNA in 70 minutes, or speed it up to 30 minutes with optional tools like the NucleoSpin Finalizer.
• High DNA Yield: Get up to 400 µg of clean plasmid DNA from each preparation—perfect for cloning, sequencing, and transfection.
• Reliable Contamination Control: The LyseControl reagent helps minimize genomic DNA contamination and ensures that your results are accurate.
• Advanced Resin Technology: The patented anion exchange resin ensures high specificity in binding plasmid DNA, leading to clean and reliable results for downstream molecular biology applications.
• User-Friendly Design: Easy to use, with a built-in column filter for smooth lysate processing and clear visual feedback from LyseControl.

Specifications

• Yield: Up to 400 µg of plasmid DNA per prep
• Time: 70 minutes per prep (reduced to 30 minutes with the NucleoSpin Finalizer)
• Technology: Silica-based anion exchange resin
• Sample Source: Plasmid DNA from E. coli cultures

Why You Should Choose This Macherey-Nagel NucleoBond Xtra Midi Kits?

If you need a quick, reliable solution for purifying plasmid DNA in your lab, the NucleoBond Xtra Midi Kit is the perfect tool. With its proven technology and high yields, it’s ideal for any molecular biology lab focused on gene cloning, sequencing, or transfection. The kit is easy to use, offers flexible options, and ensures you get clean, high-quality plasmid DNA every time.

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